Curriculum Vitae of 

Jennifer Lynn Fiedler Larimore

Associate Professor

Agnes Scott College

Chair of the Department of Biology

Co-Director of the Neuroscience Program

jllarimore@mac.com

http://www.ilovebrains.org

 

 Degrees Earned

University of Alabama at Birmingham (UAB), Birmingham Alabama         

2003-2008        

Doctorate of Philosophy            

Department of Neurobiology 

Dissertation: “The role of centaurins in vesicle trafficking and in neuronal differentiation.”

 

Asbury University, Wilmore, Kentucky                                                                     

1997-2001        

Bachelor of Arts                        

Degree Major: Biochemistry 

 

 

 

Research Experience

 Agnes Scott College, Department of Biology Decatur, Georgia

July 2012 – present

Assistant Professor

With the work of undergraduates in my lab, I am exploring the roles of BLOC-1 dependent endosomal trafficking in parvalbumin-positive GABAergic internueorn migration and localization in the hippocampus. Interestingly, subunits of the BLOC-1 complex have decreased protein levels in patients with Schizophrenia and in Autism Spectrum Disorder. Utilizing molecular neurobiology techniques, I have successfully trained undergraduates to address the research focuses of my lab. 

 

Emory University, Department of Cell Biology Atlanta, Georgia

July 2012 - present

Adjunct Faculty, in collaboration with, Victor Faundez, M.D., Ph.D.

I am exploring the role of BLOC-1 and GABAergic signaling in the neurodevelopmental disorders, Schizophrenia and Autism Spectrum Disorders. 

 

Emory University, Department of Cell Biology Atlanta, Georgia

2008-July 2012

Postdoctoral Fellow, Victor Faundez, M.D., Ph.D., mentor

I explored the role of two synaptic vesicle complexes, BLOC-1 and AP-3, in vesicle trafficking regulation from the neuronal cell body to the synapse. To do this we utilized fixed microscopy of coronal brain sections, primary cultures, and cell lines. We also prepared biochemical brain fractions to analyze protein levels by immunoblot.

 

UAB, Department of Neurobiology, Birmingham, Alabama

2004-2008 

Dissertation Research, Anne Theibert, PhD, mentor

My dissertation project used molecular biology techniques with neurosecretory PC-12 cells, primary cultured neurons, and organotypic hippocampal slice cultures to investigate the role of Arf GAPs in post-Golgi trafficking and neuronal development and differentiation. 

 

UAB, Department of Neurobiology, Birmingham, Alabama      

2006-2008

Collaboration with Chris Chapleau, Lucas Pozzo-Miller, PhD, advisor

This project examined the effects of BDNF expression and trafficking in primary cultures expressing wild type or mutant MECP2, the gene associated with Rett syndrome.

UAB, Department of Neurobiology, Birmingham Alabama      

2003-2004 

Rotation Student, Lucas Pozzo-Miller, PhD, advisor

I used live-imaging techniques to study the effects of BDNF on synaptic vesicle release in primary culture hippocampal neurons. 

Southern Research Institute, High Throughput Screening Lab, Birmingham, Alabama

2001-2003 

Research Assistant IV in High Throughput Screening, Thomas Fletcher, PhD, director

I screened approximately 3 million compounds in validation and optimization of cell-based screens with the NCI Cancer Screening Program. I also developed renovations and cell-based screens for the Department of Defense contracts in the BSL-3 facility. 

 

 

Publications

Book

J. Larimore. Neuroscience Basics: A guide to the Brain’s Involvement in Everyday Activities. Academic Press, 2017.

 Book Chapters

R. Frank, K. Edwards, J. Larimore. Chapter 19: Yoga and Pilates as Methods of Symptom Management in Multiple Sclerosis in: Watson RR, Killgore WDS, eds., Nutrition and Lifestyle in Neurological Autoimmune Diseases: Multiple Sclerosis. San Diego: Academic Press, 2017. Pp 189-194

 Manuscripts in Peer-Reviewed Journals

Jennifer Larimore, Stephanie Zlatic, Miranda Arnold, Kaela Singleton, Rebecca Cross, Hannah Rudolph, Martha Vorder Bruegge, Andrea Sweatmen, Cecilia Garza, Eli Whisnant, Victor Faundez. Dysbindin Deficiency Modifies the Expression of GABA Neuron and Ion Permeation Transcripts in the Developing Hippocampus. Frontiers in Genetics, Neurogenomics. Volume 8:28. March 2017. doi: 10.3389/fgene.2017.00028

Hannah Rudolph, Rebecca Cross, Laura Segura, Kaela Singleton, and Jennifer Larimore. Neuronal Endosomal Trafficking: One of the Common Molecular Pathways Disrupted in Autism Spectrum Disorders and Schizophrenia. J Neurol Psychol. 2016 Dec. Vol 4 Issue 2. December 2016.

Miranda Arnold, Kaela Singleton, Rebecca Cross, Christopher Chapleau, Ariana P. Mullin, Isaiah Rolle, Carlene Moore, Anne Theibert, Lucas Pozzo-Miller, Victor Faundez,  Jennifer Larimore*  The Endosome Localized ARF-GAP AGAP1 Modulates Dendritic Spine Morphology Downstream of the Neurodevelopmental Disorder Factor Dysbindin. Frontiers in Cellular Neuroscience. 2016 Sep 22. 12:218. PMID 27713690.

Gokhale A, Vrailas-Mortimer A, Larimore J, Comstra HS, Zlatic SA, Werner E, Manvich DF, Iuvone PM, Weinshenker D, Faundez V. Neuronal copper homeostasis susceptibility by genetic defects in dysbindin, a schizophrenia susceptibility factor. Hum Mol Genet. 2015 Oct 1;24(19):5512-23. doi: 10.1093/hmg/ddv282. Epub 2015 Jul 21.

Gokhale A, Mullin AP, Zlatic SA, Easley CA, Merritt ME, Raj N, Larimore J, Gordon DE, Peden AA, Sanyal S, Faundez V. The N-ethylmaleimide-sensitive factor and dysbindin interact to modulate synaptic plasticity. J Neurosci. 2015 May 13;35(19):7643-53. doi: 10.1523/JNEUROSCI.4724-14.2015.

Frank R and Larimore J. Yoga as a Method of Symptom Management in Multiple Sclerosis. Frontiers in Neurodegeneration, 30 April 2015 | http://dx.doi.org/10.3389/fnins.2015.00133.

Larimore J, Zlatic SA, Gokhale A, Tornieri K, Singleton KS, Mullin AP, Tang J, Talbot K, Faundez V. Mutations in the BLOC-1 Subunits Dysbindin and Muted Generate Divergent and Dosage-Dependent Phenotypes. J Biol Chem. 2014 Apr 8. 

Olivia Bello, Kelsey Blair, Christopher Chapleau and Jennifer L. Larimore. Is memantine a potential therapeutic for Rett Syndrome? Frontiers in Neurosciene. December 2013.

Jennifer Larimore, Pearl V. Ryder, Kun-Yong Kim, L. Alex Ambrose, Christopher Chapleau, Gaston Calfa, Christina Gross, Gary Bassell, Lucas Pozzo-Miller, Yoland Smith, Konrad Talbot, In-Hyun Park, Victor Faundez. MeCP2 Regulates the Synaptic Expression of a Dysbindin-BLOC-1 Network Component in Mouse Brain and Human Induced Pluripotent Stem Cell-Derived Neurons. PLOS-1 Biology. Accepted April 2013.

Christopher A Chapleau, Jane Lane, Jennifer Larimore, Wei Li, Lucas Pozzo-Miller, Alan K Percy. Recent progress in Rett syndrome and MECP2 dysfunction: assessment of potential treatment options. Future Neurology, Jan 2013, Vol. 8, No. 1, Pages 21-28.

Li W, Calfa G, Larimore J, Pozzo-Miller L. Activity-dependent BDNF release and TRPC signaling is impaired in hippocampal neurons of Mecp2 mutant mice. Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):17087-92. 

Gokhale, A,; Larimore, J.; Mullin, A.; So, L.; Moreno De Luca, A.; Lese-Martin, C.; Brodsky, F.; Lupashin, V., Smith, Y.; Victor Faundez. Quantitative Proteomes of a Schizophrenia Susceptibility Factor Identify Novel Roles of the Endosome-Localized BLOC-1 Complex. Journal of Neuroscience. March 2012.

Larimore, J. Tornieri, K., Ryder, P., Gokhale, A., Zlatic, S., Craige, B., Lee, J., Talbot, K., Pare, J., Smith, Y., Faundez, V. The Schizophrenia Susceptibility Factor Dysbindin and its Associated Complexes Target Membrane Cargoes to the Synapse. Molecular Biology of the Cell December 2011.

* this manuscript was highlighted by the editors and was one of the 50 most read articles in October, pre-print, for MBOC.

Mullin AP*, Gokhale A*, Larimore J*, Faundez V. Cell Biology of the BLOC-1 Complex Subunit Dysbindin, a Schizophrenia Susceptibility Gene. Mol Neurobiol. 2011 Apr 26.  These authors contributed equally.

LarimoreJ., Chapleau, C., Schanen, C., Kudo, S., Theibert, A., Percy, A., and Pozzo-Miller, L. BDNF Overexpression in Cultured Hippocampal Neurons Prevents Dendritic Atrophy Caused by Rett-Associated MECP2 Mutations. Neurobiology of Disease 2009, (34) 199-211. 

Chapleau, C., Larimore, J., Theibert, A., and Pozzo-Miller, L. Modulation of dendritic spine development and plasticity by BDNF and Vesicular Trafficking: Fundamental Roles in Neurodevelopmental Disorders associated with Mental Retardation and Autism. Journal of Neurodevelopmental Disorders 2009 Sep;1(3):185-96.

Chapleau, C., Calfa.G., Lane, M., Albertson, A., Larimore, J., Kudo, S., Armstro, D., Percy, A., Pozzo-Miller, L. Dendritic Spine Pathologies in Hippocampal Pyramidal Neurons from Rett Syndrome Brain and after Expression of Rett-Associated MECP2 Mutations.Neurobiology of Disease 2009, (35) 219-233. 

Chapleau, C. A., Carlo, M.E., Larimore, J. L. and Pozzo-Miller, L. The Actions of BDNF on Dendritic Spine Density and Morphology in Organotypic Slice Cultures Depend on the Presence of Serum in Culture Media. Journal of Neuroscience Methods 2008, 169 (1) 182-190. 

Moore, C., Thacker, E., Larimore, J., Gaston, D., Underwood, A., Kearns, B., Patterson, S., Jackson, T., Chapleau, C., Pozzo-Miller, L., Theibert, A. 2007. The neuronal Arf GAP centaurin {alpha}1 modulates dendritic differentiation. Journal of Cell Science 2007, 120:2683-2693.

 

Invited Research Talks to International Meetings

International Rett Syndrome Webinar

October 2012

“From Mind to Molecules: the Neurobiology of Rett Syndrome.”

 

International Rett Syndrome World Congress, New Orleans, LA

June 2012

“From Mind to Molecules: the Neurobiology of Rett Syndrome.”

 

American Society of Cell Biology (ASCB) Annual Meeting, Washington, D.C.

December 2007 

From my dissertation work: “The Arf GAP Centaurin γ-2 (AGAP1) modulates neuronal differentiation.” 

 

Research Funding

Professional Development Fund

$3720

April 2018 – May 2019

functional Endosomal Trafficking Kinetics in Cells expressing altered genetics observed in the neurodevelopmental disorders Rett Syndrome and Schizophrenia 

Schizophrenia and Rett Syndrome are neurodevelopmental disorders with alterations in pyramidal cell morphology in the hippocampus (the brain region necessary for learning and memory). This morphological observation has been confirmed by alterations in genes that regulate cell morphology in both disorders. This alteration in cell morphology may be due to a change in endosomal trafficking. I want to explore alterations in endosomal trafficking in N2A (mouse neuroblastoma) cells expressing mutations in genes observed in Rett Syndrome (T158M mutation in MeCP2) and Schizophrenia (knock-down of BLOC-1 subunit dysbindin).

 

Gravett Fund Award

$1800

June 2016 – May 2017

Examining the Molecular Cross Roads between Schizophrenia and Autism

Genome wide analysis studies have revealed an overlap of molecular processes disrupted in neurodevelopmental disorders such as Schizophrenia and Autism. By examining the genomic data, we will create groups of affected genes based on the function of the protein within the cell (functional groups). We will examine the data published on the functional groups and determine which proteins should be examined. Using western blot analysis to quantify the amount of protein and immunohistochemistry to examine the location of the protein, we will analyze any changes in protein levels or localization within cell models of schizophrenia and autism compared to control.

 

Buchanan Research Award

$3240

April 2016 – May 2017

Parvalbumin neuron development in the GABAergic phenotype of BLOC-1 and NRXN1 deficient mice

We will explore two mechanisms that may impact parvalbumin interneuron development in our specific aims: migration of the interneuron precursors or a decrease in the population of the parvalbumin interneurons once they have reached their target brain region. These mechanisms of potential regulation may occur independently of each other or simultaneously, and, as such, each should be considered. 

 

Holder Fund for Faculty Innovations

$4000

June 2015 – June 2016

Our lab hypothesizes that impaired BLOC-1 function contributes to the GABAergic phenotype observed in the BLOC-1 null mice. To test this, we will examine how BLOC-1 regulates the number of parvalbumin interneurons during development.Our preliminary data (Figure 1) demonstrate an alteration in the GABAergic markers at P7 in mice lacking dysbindin (Larimore et al., 2014). We will examine hippocampus and prefrontal cortex isolated from several developmental stages from mice lacking the BLOC-1 complex to compare protein and mRNA levels of GABAergic markers.

 

Buchanan Research Award

$2500

May 2015 – May 2016

Our lab hypothesizes that impaired BLOC-1 function contributes to the GABAergic phenotype observed in the BLOC-1 null mice. To test this, we will examine how BLOC-1 regulates the interneurons ability to switch from excitatory to inhibitory. Between P7 and P14 in the mouse, interneurons switch from excitatory to inhibitory due to the insertion of the KCC2 chloride channel. We will examine hippocampus and prefrontal cortex isolated from several developmental stages from mice lacking the BLOC-1 complex.  With these samples we will determine the mRNA and protein levels of the chloride channels required for proper GABA function.

 

Buchanan Research Award

May 2014 – May 2015

$4000

Our lab hypothesizes that impaired BLOC-1 function contributes to the GABAergic phenotype observed in the BLOC-1 null mice. To test this, we will examine how BLOC-1 regulates the migration of interneurons into the hippocampus. Hippocampal interneurons arise from the medial ganglionic eminence and cadual ganglionic eminence and are produced during E9-12 and E-12-E16 respectively. It is possible that BLOC-1 regulates the production or the migration of these neurons into the hippocampus between E13-E16. Using immunofluorescent confocal microscopy to examine the hippocampi isolated from several developmental stages of mice lacking the BLOC-1 complex and determine if interneuron migration is occurring properly.

 

Net/WORK, a BP-EDURE blueprint NIH grant, Agnes Scott Director

$100,000 a year for three years. 

June 2012 – August 2015

The fellowship mentors under-represented undergraduates in research laboratories during the Junior and Senior year. For students from underrepresented groups at Georgia State University, Emory University, Spelman College, and Agnes Scott College, NET/work is a two-year paid research assistantship that provides participants with local and national research opportunities from their sophomore year through graduation.

 

International Rett Syndrome Foundation Fellowship 

$50,000 a year for two years.

September 2012- August 2014

This grant explored the transcriptional regulation of BLOC-1 complex subunits by MeCP2, a gene product whose function is impaired in Rett syndrome. This study resulted in key information about the role of BLOC-1 in the synaptic pathogenic mechanisms that characterize Rett syndrome and schizophrenia.

 

FIRST Fellowship, Emory University 

$44,000 each year for three years.

2009-2012

The Fellowship provided training in research as well as science education. The Fellowship required both a science mentor and a teaching mentor. Through this fellowship, I was mentored in the lab of Victor Faundez for research training, which resulted in four publications, two as first author. I chose a teaching mentor at Spelman College, Kai McCormick, Ph.D. Dr. McCormick trained me in active learning techniques and pedagogical practices that engage students while we co-taught her Brain and Behavior class at Spelman.

 

Civitan International Emerging Scholars Grant 

$25,000 for one year.

2005-2006 

This funding part of my dissertation research. This award investigated the role of vesicular trafficking regulated by ArfGAPs disrupted in Autism Spectrum Disorder. 

 

Teaching Experience

 

Agnes Scott College, Decatur, Georgia

Fall 2012  - present

BIO 110: Integrative Biology I

BIO 191: Genetics and Molecular Biology

BIO 210: Scientific Inquiry and Communication

BIO 250 and 251: Foundations in Neuroscience 1 &2

BIO 316: Molecular Biology

BIO 330: Diseases of the nervous system.

BIO 380: Directed Research

BIO 410: Independent Study

BIO 440: Directed Research

BIO 450: Directed Internship

BIO 490: Senior Honors Thesis

BIO 492: Senior Seminar in Learning and Memory

Mentored research experiences (BIO 380, 410, 440, 450, and 490) have resulted in various publications and presentations. Students that I have mentored in these experiences are listed on the following page with the outcomes of those experiences.

 

Spelman College, Atlanta, Georgia

Fall 2010

Co-Instructor, Kai McCormack, Ph.D., teaching mentor

Brain and Behavior, Psychology 320: Required coursework for majors in the Psychology Department.

 

University of Alabama at Birmingham, Birmingham, Alabama

2005-2008


Mentor, Graduate Rotation Students

Braden Cox                  Fall 2005

Sean Markwardt            Winter 2006-2007

Sharday Ewell               Spring 2008      

 

Mentor, Undergraduate Summer Research Projects

David Gaston                2005

Allison Smith                2006

Isaiah Rolle                  200


 

English Instructor, Kibera Primer School, Kiberia, Kenya, West Africa

2001, 2006, 2007

English teacher for a one-week module on grammar, spelling, and pronunciation.

 

Asbury University, Wilmore, Kentucky 

1998-2001

Lab assistant, freshman non-Major’s Biology laboratory sections.

 

The Birmingham Swim League, Birmingham, Alabama

1993-2003

Swim coach for elite, competitive swimmers.

Undergraduates mentored in Research at Agnes Scott College, Decatur GA

 NameTime in Lab. Outcome of research in lab

 

Karima Johnson . Fall 2012 – Spring 2013. SpARC poster presentation

Leila IbrahimFall 2012 – Spring 2013. SpARC poster presentation

Laurel (Alex) AmbroseFall 2012 – December 2013. SpARC poster presentation *published manuscript

 Jessica Resnack Fall 2013 – May 2014

 Olivia BelloSpring 2014. SpARC oral presentation   *published manuscript

 Pamela GuinnSpring 2014. SpARC oral presentation

 Jasmine SpragginsSpring 2014. SpARC oral presentation

 Kaela SingletonFall 2013 – Summer 2014. SpARC oral presentation  *published 2 manuscripts

 Laura Segura. Spring 2014 – Summer 2014. SpARC oral presentation

 Katherine BinkowskiSummer 2014-Fall 2015

 Rebecca Cross. Spring 2014 – Spring 2015. SpARC oral presentation *published manuscript*Honor’s Thesis

 Eli WhistantSpring 2015. SpARC oral presentation *published manuscript

 Cecilia GrazaSpring 2015. SpARC oral presentation *published manuscript

 Kathryn LapradeSpring 2015                           

 Martha VoderBruggeSpring 2015. SpARC oral presentation *published manuscript

 Nandi Rameriez Spring 2015. SpARC oral presentation

 Andi Sweatman Spring 2015. SpARC oral presentation *published manuscript

 Miranda Arnold Summer 2015 – Spring 2017. Society for Neuroscience, Poster (2015) and talk (2016) * published 2 manuscripts and Honor’s Thesis

 Megan DoFall 2016

 Tempist HarrisFall 2016

 Alveena Jacks. Fall 2016 – Spring 2017. SpARC oral presentation

 Karina Leung. Spring 2017 – Spring 2018. SpARC oral presentation

 Anner HarrisSpring 2017 – Spring 2018. SpARC oral presentation

 Hannah Rudolph. Spring 2015 – Spring 2017. SpARC oral presentation, Society for Neuroscience, Poster Presentations, Honor’s Thesis and *published 2 manuscripts

 Roysheda TarverFall 2017. Scotties with Nerves, Oral Presentation

 Crystal Burgess Fall 2017 – Spring 2018. SpARC Oral Presentation and Scotties with Nerves, Oral Presentation and an Honor’s Thesis

Susan Cordero Romero. Spring 2018 – Spring 2019. SpARC Oral Presentation, Scotties with Nerves, Oral Presentation, Society for Neuroscience, Poster Presentation and an Honor’s Thesis.

Jhodi WebsterSummer 2018 – present. GF-STEM Symposium, Society for Neuroscience, Poster 2018, Society for Neuroscience, Poster 2019, ABRCMS poster presentation 2019, and GURC presentation 2019

 Alexia Crockett Summer 2018. GF-STEM Symposium

 Ruvimbo DzvurumiSummer 2018 – present. GF-STEM Symposium, Society for Neuroscience, Poster 2018, ABRCMS poster presentation 2019, GURC presentation 2019.

 Lauren NealSpring 2018 – present. SpARC Oral Presentation, Society for Neuroscience, Poster 2018, Society for Neuroscience, Poster 2019, ABRCMS poster presentation 2019, GURC presentation 2019       

 Alex Lombardo .Summer 2019 – present . Summer symposium presentation, Society for Neuroscience, Poster Presentation, GURC presentation 2019

 Sammi Hatcher. Summer 2019. Summer Symposium presentation

 Alix Wagner (High School Student)Summer 2019.Summer Symposium presentation

 Rachel ThompsonSummer 2019 – present. Society for Neuroscience, Poster Presentation, ABRCMS poster presentation 2019

 Camryn SmithFall 2019. Society for Neuroscience, Poster 2019